Dataset details
Technique | LCM-seq |
---|---|
Title | Topographical transcriptome mapping of the mouse medial ganglionic eminence by spatially resolved RNA-seq |
PMID | 25344199 |
Description | This study adapted the STRT (single-cell tagged reverse transcription) method to laser microdissected tissue samples. By systematically sampling the mouse medial ganglionic eminence (MGE) tissues in a regular grid, they isolated 50×50×50 μm3 cubes that are akin to the `voxels’ in a three-dimensional volumetric space. |
Source | wild-type and Gfra1Tlz mutant embryos (both C57bl6/J) |
Species | mouse |
No. genes | 14942 genes |
No. cells | NA |
Resolution | 50 um |
Data normalization | TPM |
Data availability | GSE60402 |