| Technique |
LCM-seq |
| Title |
Laser capture microscopy coupled with Smart-seq2 for precise spatial transcriptomic profiling |
| PMID |
27387371 |
| Description |
This study applied LCM-seq on neurons isolated from mouse tissues, human post-mortem tissues, and illustrate its utility down to single captured cells. |
| Source |
spinal cords from five-day-old mouse pups (n=7) (males and females), brains were dissected from 8-week-old female mice (n=3, two different litters), mice (B6 and FVB, wild-type, from Jackson Laboratories stock numbers 004435 and 005052); fresh frozen human post-mortem tissues (spinal cord and brain) |
| Species |
human and mouse |
| No. genes |
28776 genes (mouse), 33704 genes (human) |
| No. cells |
NA |
| Resolution |
cellular |
| Data normalization |
FPKM |
| Data availability |
GSE76514 |