Dataset details
| Technique | MERFISH |
|---|---|
| Title | Molecular, spatial, and functional single-cell profiling of the hypothalamic preoptic region |
| PMID | 30385464 |
| Description | This study developed an imaging-based in situ cell-type identification and mapping method (MERFISH) and combined it with single-cell RNA-sequencing to create a molecularly annotated and spatially resolved cell atlas of the mouse hypothalamic preoptic region. |
| Source | brain slices from adult C57Bl6/J male and female mice (aged 7-8 weeks) |
| Species | mouse |
| No. genes | 155 genes |
| No. cells | 1000000 cells |
| Resolution | single molecule |
| Data normalization | Quantile normalization |
| Data availability | scRNA-seq data are available at Gene Expression Omnibus (GEO) (GSE113576). MERFISH data are available at Dryad, and the analysis software is available at https://github.com/ZhuangLab/MERFISH_analysis. |
Spatially variable genes identified by SpatialDE (Q-value<0.05)
| ID | Gene | Species | Sample | P-value | Q-value |
|---|---|---|---|---|---|
| ID | Gene | Species | Sample | P-value | Q-value |