| Technique |
seqFISH |
| Title |
Identification of spatially associated subpopulations by combining scRNAseq and sequentialfluorescence in situ hybridization data |
| PMID |
30371680 |
| Description |
Seq-FISH combined scRNA-seq was applied to dissect the cell-type- and spatial-domain-associated heterogeneity in the mouse visual cortex region. |
| Source |
brain sections (visual cortex region) from C57BL/6 with Ai6Cre-reporter (uncrossed) female mice aged 50-80 days |
| Species |
mouse |
| No. genes |
125 genes |
| No. cells |
1597 cells |
| Resolution |
single molecule |
| Data normalization |
- |
| Data availability |
Expression data, spatial coordinates, SVM predictions, HMRF domains, expression box plots categorized by domains and cell types, and interactive visualizations are available at http://spatial.rc.fas.harvard.edu. The scRNA-seq dataset referenced in this study is GSE71585. |