| Technique |
seqFISH |
| Title |
Transcriptome-scale super-resolved imaging in tissues by RNA seqFISH+ |
| PMID |
30911168 |
| Description |
SeqFISH+ can image mRNAs for 10,000 genes in single cells-with high accuracy and sub-diffraction-limit resolution-in the cortex, subventricular zone and olfactory bulb of mouse brain, using a standard confocal microscope. |
| Source |
Wild-type mice C57BL/6J of post-natal day (P)23 (male) and P40 (male) were used for the cortex and olfactory bulb seqFISH+. |
| Species |
mouse |
| No. genes |
10,000 genes |
| No. cells |
913 cortex cells, 2050 olfactory bulb cells |
| Resolution |
single molecule |
| Data normalization |
- |
| Data availability |
RNA-seq data were obtained from GEO accession number GSE98674. RNA SPOTs data were obtained from a previous study. Source data are available at https://github.com/CaiGroup/seqFISH-PLUS. |