Technique |
Spatial transcriptomics |
Title |
Visualization and analysis of gene expression in tissue sections by spatial transcriptomics |
PMID |
27365449 |
Description |
By positioning histological sections on arrayed reverse transcription primers with unique positional barcodes, this study demonstrated high-quality RNA-sequencing data with maintained two-dimensional positional information from the mouse brain and human breast cancer. |
Source |
adult C57BL/6 mouse (>2 months old) olfactory bulb, breast cancer biopsy |
Species |
human and mouse |
No. genes |
19025 genes (mouse), 16385 genes (human) |
No. cells |
NA |
Resolution |
100 um |
Data normalization |
Median ratio normalization |
Data availability |
Gene counts and scripts can be downloaded from www.spatialtranscriptomicsresearch.org. |