Details
| Technique | Tomo-seq |
|---|---|
| Title | Sequencing mRNA from cryo-sliced drosophila embryos to determine genome-wide spatial patterns of gene expression |
| PMID | 23951250 |
| Description | Individual blastoderm stage Drosophila melanogaster embryos along the anterior-posterior axis were cryosectioned and the mRNA isolated from each 25 µm slice was sequenced. A genome-wide timecourse of spatial gene expression from shortly after fertilization through gastrulation was generated. |
| Source | frozen embryo slices |
| Species | drosophila |
| No. genes | 15742 genes |
| No. cells | NA |
| Resolution | cellular |
| Data normalization | FPKM |
| Data availability | GSE43506 |