Details
| Technique | Tomo-seq | 
|---|---|
| Title | Genome-wide RNA tomography in the zebrafish embryo | 
| PMID | 25417113 | 
| Description | This study combined traditional histological techniques with low-input RNA sequencing and mathematical image reconstruction to generate a high-resolution genome-wide 3D atlas of gene expression in the zebrafish embryo at three developmental stages. | 
| Source | the yolk and straightened zebrafish embryos at the 15 somites stage (fish lines: TL, Tg(h2afva:h2afva-mCherry)) | 
| Species | zebrafish | 
| No. genes | 14254 genes | 
| No. cells | NA | 
| Resolution | cellular | 
| Data normalization | Spike-in normalization | 
| Data availability | GSE59873 |